To systematically review the development of research on human papillomavirus (HPV) infection and next-generation sequencing (NGS) technology, identify research hotspots, thematic evolution, and future trends, and provide a reference for future scientific research topics and clinical translation.

Using the PubMed database as the data source, English-language articles (type: article) published between June 2016 and June 2026 on HPV infection and NGS were retrieved, yielding a total of 69 articles. The citexs platform was used to conduct bibliometric analysis of annual publication output, countries/regions, institutions, authors, journals, keywords, associated genes, and disease distributions. Visualized maps were generated to illustrate research hotspots and changing trends.

① Annual publication output showed an overall upward trend, with an average of 6.3 articles per year and a peak of 14 articles in 2021 (a year-on-year increase of 250%), indicating rapid development in this field.

② The top three countries in publication output were China (11 articles, 15.94%), the United States (11 articles, 15.94%), and Brazil (7 articles, 10.14%). The institutions with the most publications were Aarhus University, Chulalongkorn University, and Huazhong University of Science and Technology (3 articles each).

③ Prolific authors included Benedicte Ulhøi, Ding Ma, and Esmeralda A Soares (2 articles each).

④ The journals with the most publications were Archives of Virology (3 articles) and Viruses (3 articles), followed by BMC Cancer (2 articles).

⑤The high-frequency keywords were human papillomavirus, hpv, ngs, next-generation sequencing, and cervical cancer, reflecting that research focuses on HPV infection detection, NGS technology application, and their relationship with cervical cancer.

⑥Gene analysis showed that CDKN2A and TP53 were the most frequently involved genes (18 articles each), suggesting their key roles in HPV-related tumorigenesis. For disease analysis, Papillomavirus Infections (52 articles), Neoplasms (48 articles), and Uterine Cervical Neoplasms (47 articles) were the main associated diseases.

Research on HPV infection and NGS has developed rapidly over the past decade, with a focus on early screening of cervical cancer, viral integration, and host gene mutation detection. NGS technology has significantly improved the precision of HPV genotyping and the study of oncogenic mechanisms. However, current research faces issues such as imbalanced international distribution and insufficient institutional collaboration. Future efforts should strengthen multicenter cooperation, deepen multi-omics integrated research on HPV, and promote the application of NGS in personalized clinical diagnosis and treatment.